• Research Assistant Professor
• Campus Address:  MS 420
• Telephone: (317) 274-7872
• E-mail:  dyan@iupui.edu

Training

• B.S., 1988: Beijing Agricultural University, Beijing, China
• Ph.D., 1993: Beijing Agricultural University, Beijing, China
• Postdoctoral, 1997: University of California at Berkeley, California

Research Summary

Nitrogen assimilation is a major branch of cellular metabolism.  The central nitrogen metabolic circuit in enteric bacteria is a compact system consisting of limited number of key enzymes and central intermediate metabolites.  This circuit is however intensively regulated by multiple regulatory cascades responding to cellular nitrogen and carbon status.  Using E. coli as the model organism, the research in my laboratory is focused on the control of nitrogen assimilation and its coordination with carbon metabolism.

Publications

Yan, D.  Protection of the glutamate pool concentration in enteric bacteria. Proc Natl Acad Sci U S A 104: 9475-9480, 2007.
 
Lee, S. Y., De La Torre, A., Yan, D., Kustu, S., Nixon, B. T., and Wemmer, D. E.  Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains. Genes Dev. 17: 2552-2563, 2003.

Cho, H., Wang, W., Kim, R., Yokota, H., Damo, S., Kim, S. H., Wemmer, D., Kustu, S., and Yan, D.  BeF3- acts as a phosphate analog in proteins phosphorylated on aspartate: structure of a BeF3- complex with phosphoserine phosphatase. Proc Natl Acad Sci U S A 98: 8525-8530, 2001.

Yan, D., Cho, H. S., Hastings, C. A., Igo, M. M., Lee, S. Y., Pelton, J. G., Stewart, V., Wemmer, D. E., and Kustu, S.  Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proc Natl Acad Sci U S A 96: 14789-14794, 1999.

Yan, D., Ikeda, T. P., Shauger, A. E., and Kustu, S.  Glutamate is required to maintain the steady-state potassium pool in Salmonella typhimurium. Proc Natl Acad Sci U S A 93: 6527-6531, 1996.